Inactivation of Icmt inhibits transformation by oncogenic K-Ras and B-Raf
J. Clin. Invest. Martin O. Bergo, et al. 113:539 doi:10.1172/JCI18829 [Go to this article.]

Figure 4
Impaired ability of K-Ras–transfected Icmt-deficient fibroblasts to contribute to tumor growth in nude mice. A mixed population of K-Ras-Icmt^flx/flx cells and derivative K-Ras-Icmt^Δ/Δ cells (total of 500,000 cells, prepared as described in the Methods section) was injected subcutaneously into nude mice. Tumors were harvested 21 days later. Southern blots were performed on genomic DNA prepared from the injected cells and the tumors. The ratio of Icmt^flx to Icmt^Δ band intensity was much greater in the tumor DNA than in the injected cells. A 5-kb Icmt⁺ allele in some lanes was due to host DNA in the tumors.