Critical role of IL-10 in the induction of low zone tolerance to contact allergens
J. Clin. Invest. Marcus Maurer, et al. 112:432 doi:10.1172/JCI18106 [Go to this article.]

Figure 2
The development of LZT effector T cells is IL-10 dependent. Mice were treated with solvent or tolerizing doses of TNCB (0.45 or 4.5 μg) and subsequently sensitized and challenged. After 24 hours, LNCs from (a) IL-10^+/+, IL-10^–/–, or IL-10^–/– mice reconstituted with IL-10^–/– or (b) whole LNCs or LNCs depleted of CD8⁺ or CD4⁺ T cells from IL-10^+/+ mice were obtained to measure the release of IL-4 (Tc2/Th2 cytokine) and IFN-γ (Tc1/Th1 cytokine) by ELISA (48 hours after hapten-specific restimulation). (c) LNCs were isolated 24 hours after challenge from IL-10^+/+, IL-10^–/–, or IL-10^–/– mice reconstituted with IL-10 and treated as described above. Thirty-six hours after hapten-specific restimulation, the proliferation of LNCs was measured by incorporation of [³H]thymidine. In each case, one of three independent experiments with similar results is shown. (d) In some experiments LNCs or T cells isolated from LNCs (> 95% purity) 24 hours after tolerization or treatment with solvent were transferred to untreated mice (one single intravenous injection of 5 × 10⁷ LNCs or 1 × 10⁷ T cells per mouse), and expression of LZT in these animals was assessed after sensitization and challenge by measuring ear-swelling responses.