Critical role of the Toll-like receptor signal adaptor protein MyD88 in acute allograft rejection
J. Clin. Invest. Daniel R. Goldstein, et al. 111:1571 doi:10.1172/JCI17573 [Go to this article.]

Figure 2
MyD88^–/– recipients have a reduced number of mature and immature DCs in lymph nodes draining the allografts. (a) Despite having similar numbers of mature (CD40⁺CD11c⁺, CD80⁺CD11c⁺, and CD86⁺CD11c⁺) DCs on day 0, MyD88^–/– recipients were unable to increase numbers of mature DCs on days 7 and 14, whereas WT counterparts had stable numbers of mature DCs during the first week that increased nearly fivefold by day 14 (P = 0.0001 vs. MyD88^–/–). (b) Immature (CD40^–CD11c⁺, CD80^–CD11c⁺, and CD86^–CD11c⁺) DCs failed to accumulate in the draining lymph nodes of allografts of MyD88^–/– recipients of MyD88^–/– male grafts on days 0, 7, and 14 after transplantation (on day 14, MyD88^+/+ CD40^–CD11c⁺ vs. MyD88^–/– CD40^–CD11c⁺ P = 0.0002; MyD88^+/+ CD80^–CD11c⁺ vs. MyD88^–/– CD80^–CD11c⁺ P < 0.01; MyD88^+/+ CD86^–CD11c⁺ vs. MyD88^–/– CD86^–CD11c⁺ P < 0.01). (c) Analysis of the number of mature DCs in the draining lymph nodes in different transplant combinations on day 14 after transplantation. The presence of WT APCs in either the donor or the recipient partially restored the number of mature DCs present in the draining lymph nodes when compared with the MyD88^+/+ → MyD88^+/+ transplant combination. *P < 0.009 vs. MyD88^+/+ → MyD88^+/+, P < 0.02 vs. MyD88^–/– → MyD88^–/–; **P < 0.02 vs. MyD88^+/+ → MyD88^+/+, P < 0.006 vs. MyD88^–/– → MyD88^–/–.