WNK kinases regulate thiazide-sensitive Na-Cl cotransport
J. Clin. Invest. Chao-Ling Yang, et al. 111:1039 doi:10.1172/JCI17443 [Go to this article.]

Figure 2
(a) Immunoblot of immunoprecipitated oocyte membranes probed with anti-NCC antibody. Oocytes injected with NCC, NCC + WNK4, and NCC + WNK4 + WNK1 express the same amount of core-glycosylated protein. The ratio of mature glycosylated to core-glycosylated NCC is the same in all three groups. (b) Immunocytochemistry of oocytes stained with anti-NCC. Representative oocytes injected with NCC, NCC + WNK4, and NCC + WNK4 + WNK1 are shown. (c) Immunoblot of surface-biotinylated NCC. Only the mature glycosylated form of NCC was evident in this blot (data not shown). Coexpression of WNK4 with NCC reduced surface NCC expression by 85% (see Results). Coexpression with WNK1 and WNK4 restored surface NCC levels toward levels observed with NCC alone.