α1-adrenergic receptors activate Ca²⁺-permeable cationic channels in prostate cancer epithelial cells
J. Clin. Invest. Stephanie Thebault, et al. 111:1691 doi:10.1172/JCI16293 [Go to this article.]

Figure 3
Membrane-permeable DAG analogues induce [Ca²⁺]_i signaling in LNCaP cells. (a) [Ca²⁺]_i oscillations in a representative LNCaP cell evoked by the membrane-permeable DAG analogue OAG (100 μM, n = 37). (b) OAG-evoked [Ca²⁺]_i oscillations in a LNCaP cell disappear in nominally Ca²⁺-free medium (0/Ca²⁺, n = 45). (c and d) Coapplications of cationic channel blockers SK&F 96365 (10 μM, n = 15) (c) and 2-APB (100 μM, n = 24) (d) suppress OAG-evoked [Ca²⁺]_i oscillations in LNCaP cells. (e) Representative transient [Ca²⁺]_i increase induced in a LNCaP cell by another membrane-permeable DAG analogue, DOG (100 μM, n = 23). [Ca²⁺]_i signals were measured on Fura-2–loaded cells; all interventions on each panel are marked by horizontal bars.