α1-adrenergic receptors activate Ca²⁺-permeable cationic channels in prostate cancer epithelial cells
J. Clin. Invest. Stephanie Thebault, et al. 111:1691 doi:10.1172/JCI16293 [Go to this article.]

Figure 2
α1A-AR agonist–evoked Ca²⁺ signaling in human prostate epithelial cells. (a and b) Changes in [Ca²⁺]_i in representative primary human prostate epithelial cells in response to Phe (10 μM, n = 17) (a) alone and (b) in combination with SK&F 96365 (10 μM, n = 9). (c) Phe-evoked changes in [Ca²⁺]_i in representative LNCaP cells under control conditions (n = 37) and in the presence of the α1-AR antagonists prazosin (n = 31) or WB4101 (n = 33) (both at 1 μM). (d) Phe-evoked [Ca²⁺]_i increase in LNCaP cells at 2 mM extracellular Ca2+ (2/Ca²⁺) and the decrease on removal of extracellular Ca²⁺ (0/Ca²⁺) (n = 29). (e) Blockade of Phe-evoked [Ca²⁺]_i increase in two representative LNCaP cells by SK&F 96365 (10 μM, n = 10) and 2-APB (100 μM, n = 10). [Ca²⁺]_i signals were measured on Fura-2–loaded cells; all interventions in each panel are marked by horizontal bars.