Impaired Smad7-Smurf–mediated negative regulation of TGF-β signaling in scleroderma fibroblasts
J. Clin. Invest. Yoshihide Asano, et al. 113:253 doi:10.1172/JCI16269 [Go to this article.]

Figure 1
Expression levels of TβRI, TβRII, Smad2, Smad3, Smad4, and Smad7 in normal and scleroderma fibroblasts. (a) Whole-cell lysates were electrophoresed through a 10% polyacrylamide gel, transferred to a nitrocellulose membrane, and probed with the indicated Ab’s. One representative of five independent experiments is shown. (b) The protein levels quantitated by scanning densitometry are shown relative to those in normal fibroblasts (100 arbitrary units [AU]). Data are expressed as the mean ± SD of five independent experiments. NS, normal skin fibroblasts; SSc, scleroderma fibroblasts.