Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration
J. Clin. Invest. Susan M. Majka, et al. 111:71 doi:10.1172/JCI16157 [
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Figure 4Vascular progenitors within skeletal muscle are derived from bone marrow. SP cells were isolated from bone marrow of Rosa26 mice and stably engrafted into lethally irradiated, genetically matched recipients. After 5 or 12 months, the hindlimb musculature was excised and examined for the presence of LacZ-positive vascular progenitors. Percoll-fractionated muscle cells were stained with Hoechst dye and loaded with FDG, then subjected to FACS to isolate muscle SP and non-SP populations and determine whether they exhibit β-gal activity. (
a) The mean ± SD of LacZ-positive SP and non-SP cells among three mice at 5 months after transplant was 39.0% ± 16.4% and 10.6% ± 1.9%, respectively. (
b) At 12 months after transplantation, the mean ± SD of LacZ-positive SP and non-SP cells among three mice was 89.4% ± 8.2% and 87.5% ± 5.2%, respectively.
