Normal breast milk limits the development of colitis in IL-10–deficient mice
KL Madsen, RN Fedorak, MM Tavernini… - Inflammatory bowel …, 2002 - academic.oup.com
KL Madsen, RN Fedorak, MM Tavernini, JS Doyle
Inflammatory bowel diseases, 2002•academic.oup.comSummary Background and Aims This study examined the role of breast milk in neonatal
bacterial colonization of the colon and disease progression in IL-10–deficient mice. Methods
IL-10–deficient mice were cross-fostered at birth and raised until weaning with a normal
mother. Results were compared with normal pups cross-fostered to an IL-10–deficient
mother. Mice were examined at various ages for histologic disease, levels of colonic
bacteria, and proinflammatory cytokine secretion. Results IL-10–deficient mice that had …
bacterial colonization of the colon and disease progression in IL-10–deficient mice. Methods
IL-10–deficient mice were cross-fostered at birth and raised until weaning with a normal
mother. Results were compared with normal pups cross-fostered to an IL-10–deficient
mother. Mice were examined at various ages for histologic disease, levels of colonic
bacteria, and proinflammatory cytokine secretion. Results IL-10–deficient mice that had …
Background and Aims
This study examined the role of breast milk in neonatal bacterial colonization of the colon and disease progression in IL-10–deficient mice.
Methods
IL-10–deficient mice were cross-fostered at birth and raised until weaning with a normal mother. Results were compared with normal pups cross-fostered to an IL-10–deficient mother. Mice were examined at various ages for histologic disease, levels of colonic bacteria, and proinflammatory cytokine secretion.
Results
IL-10–deficient mice that had been cross-fostered to a normal mother demonstrated normal levels of colonic adherent bacteria and reduced TNFα and IFNγ secretion at 2 to 12 weeks of age. Histologic disease was significantly reduced up to 12 weeks of age. Normal mice cross-fostered to an IL-10–deficient mother had increased levels of adherent bacteria at 2 and 4 weeks and increased IFNγ secretion. This group also demonstrated slight inflammation up until 12 weeks of age.
Conclusion
Breast milk has a role in neonatal bacterial colonization. Changing the luminal environment of IL-10–deficient mice during the neonatal period alters the natural disease course.
Oxford University Press