Sequence-specific DNA binding and transcription factor phosphorylation by Ku autoantigen/DNA-dependent protein kinase: phosphorylation of Ser-527 of the rat …
W Giffin, J Kwast-Welfeld, DJ Rodda… - Journal of Biological …, 1997 - jbc.org
NRE1 is a DNA sequence element through which Ku antigen/DNA-dependent protein
kinase (DNA-PK) catalytic subunit represses the induction of mouse mammary tumor virus
transcription by glucocorticoids. Although Ku is an avid binder of DNA ends and has the
ability to translocate along DNA, we report that direct sequence-specific Ku binding occurs
with higher affinity (K d= 0.84±0.24 nM) than DNA end binding. Comparison of Ku binding to
several sequences over which Ku can accumulate revealed two classes of sequence …
kinase (DNA-PK) catalytic subunit represses the induction of mouse mammary tumor virus
transcription by glucocorticoids. Although Ku is an avid binder of DNA ends and has the
ability to translocate along DNA, we report that direct sequence-specific Ku binding occurs
with higher affinity (K d= 0.84±0.24 nM) than DNA end binding. Comparison of Ku binding to
several sequences over which Ku can accumulate revealed two classes of sequence …
