Respiratory syncytial virus infection activates STAT signaling in human epithelial cells
X Kong, H San Juan, M Kumar, AK Behera… - Biochemical and …, 2003 - Elsevier
X Kong, H San Juan, M Kumar, AK Behera, A Mohapatra, GR Hellermann, S Mane…
Biochemical and Biophysical Research Communications, 2003•ElsevierAcute respiratory syncytial virus (RSV) infection causes airway inflammation and
exacerbates asthma, but the mechanism of inflammation is poorly understood. The role of
the STAT-signaling pathway in RSV infection in epithelial cells was examined in this study.
DNA microarray analyses of RSV-infected human alveolar type II (A549) epithelial cells
identified several genes whose expression was altered from− 5.5 to+ 56.4-fold. Four of the
highly expressed genes contained STAT-binding elements. In A549 and normal human …
exacerbates asthma, but the mechanism of inflammation is poorly understood. The role of
the STAT-signaling pathway in RSV infection in epithelial cells was examined in this study.
DNA microarray analyses of RSV-infected human alveolar type II (A549) epithelial cells
identified several genes whose expression was altered from− 5.5 to+ 56.4-fold. Four of the
highly expressed genes contained STAT-binding elements. In A549 and normal human …
Acute respiratory syncytial virus (RSV) infection causes airway inflammation and exacerbates asthma, but the mechanism of inflammation is poorly understood. The role of the STAT-signaling pathway in RSV infection in epithelial cells was examined in this study. DNA microarray analyses of RSV-infected human alveolar type II (A549) epithelial cells identified several genes whose expression was altered from −5.5 to +56.4-fold. Four of the highly expressed genes contained STAT-binding elements. In A549 and normal human bronchial epithelial cells (NHBE), RSV induced phosphorylation and nuclear translocation of STAT-1α that was abrogated when RSV attachment was blocked. Treatment with a JAK-2 inhibitor or transfection with dominant-negative STAT-1α blocked STAT-1α activation and RSV infection. RSV also activated STAT-3 and IL-6 specific antibodies blocked this activation. Thus, activation of the STAT-1α and STAT-3 pathways play a role in RSV infection.
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