[HTML][HTML] Targeted profiling of circulating and hepatic bile acids in human, mouse, and rat using a UPLC-MRM-MS-validated method
JC García-Cañaveras, MT Donato, JV Castell… - Journal of lipid …, 2012 - ASBMB
Bile acids (BAs) are a group of chemically related steroids recognized as regulatory
molecules whose profiles can change in different physio-pathological situations. We have
developed a sensitive, fast, and reproducible ultraperformance liquid chromatography/
multiple reaction monitoring/mass spectrometry method to determine the tissue and sera BA
profiles in different species (human, rat, and mouse) by quantifying 31 major and minor BA
species in a single 21-min run. The method has been validated according to FDA …
molecules whose profiles can change in different physio-pathological situations. We have
developed a sensitive, fast, and reproducible ultraperformance liquid chromatography/
multiple reaction monitoring/mass spectrometry method to determine the tissue and sera BA
profiles in different species (human, rat, and mouse) by quantifying 31 major and minor BA
species in a single 21-min run. The method has been validated according to FDA …