Synthesis of anti‐reverse cap analogs (ARCAs) and their applications in mRNA translation and stability
E Grudzien‐Nogalska, J Stepinski, J Jemielity… - Methods in …, 2007 - Elsevier
Synthetic capped RNA transcripts produced by in vitro transcription in the presence of m 7
Gp 3 G have found a wide application in studying such processes as mRNA translation, pre‐
mRNA splicing, mRNA turnover, and intracellular transport of mRNA and snRNA. However,
because of the presence of a 3′‐OH on both m 7 Guo and Guo moieties of the cap
structure, one‐third to one‐half of the mRNAs contain a cap incorporated in the reverse
orientation. The reverse cap structures bind poorly to eIF4E, the cap binding protein, and …
Gp 3 G have found a wide application in studying such processes as mRNA translation, pre‐
mRNA splicing, mRNA turnover, and intracellular transport of mRNA and snRNA. However,
because of the presence of a 3′‐OH on both m 7 Guo and Guo moieties of the cap
structure, one‐third to one‐half of the mRNAs contain a cap incorporated in the reverse
orientation. The reverse cap structures bind poorly to eIF4E, the cap binding protein, and …