Hyaluronan-CD44 interaction with Rac1-dependent protein kinase N-γ promotes phospholipase Cγ1 activation, Ca2+ signaling, and cortactin-cytoskeleton function …
LYW Bourguignon, PA Singleton, F Diedrich - Journal of Biological …, 2004 - jbc.org
In this study we have investigated hyaluronan (HA)-CD44 interaction with protein kinase N-γ
(PKNγ), a small GTPase (Rac1)-activated serine/threonine kinase in human keratinocytes.
By using a variety of biochemical and molecular biological techniques, we have determined
that CD44 and PKNγ kinase (molecular mass∼ 120 kDa) are physically linked in vivo. The
binding of HA to keratinocytes promotes PKNγ kinase recruitment into a complex with CD44
and subsequently stimulates Rac1-mediated PKNγ kinase activity. The Rac1-activated …
(PKNγ), a small GTPase (Rac1)-activated serine/threonine kinase in human keratinocytes.
By using a variety of biochemical and molecular biological techniques, we have determined
that CD44 and PKNγ kinase (molecular mass∼ 120 kDa) are physically linked in vivo. The
binding of HA to keratinocytes promotes PKNγ kinase recruitment into a complex with CD44
and subsequently stimulates Rac1-mediated PKNγ kinase activity. The Rac1-activated …