Silencing of SPC2 expression using an engineered δ ribozyme in the mouse βTC-3 endocrine cell line
F D'Anjou, LJ Bergeron, NB Larbi, I Fournier… - Journal of Biological …, 2004 - jbc.org
Endoproteolytic processing is carried out by subtilase-like pro-protein convertases in
mammalian cells. In order to understand the distinct roles of a member of this family (SPC2),
gene silencing in cultured cells is an ideal approach. Previous studies showed limited
success in either the degree of inhibition obtained or the stability of the cell lines. Here we
demonstrate the high potential of δ ribozyme as a post-transcriptional gene silencing tool in
cultured cells. We used an expression vector based on the RNA polymerase III promoter to …
mammalian cells. In order to understand the distinct roles of a member of this family (SPC2),
gene silencing in cultured cells is an ideal approach. Previous studies showed limited
success in either the degree of inhibition obtained or the stability of the cell lines. Here we
demonstrate the high potential of δ ribozyme as a post-transcriptional gene silencing tool in
cultured cells. We used an expression vector based on the RNA polymerase III promoter to …