Background Recent single-cell RNA sequencing (scRNA-seq) analyses have offered much
insight into cell-specific gene expression profiles in normal kidneys. However, in diseased
kidneys, understanding of changes in specific cells, particularly glomerular cells, remains
limited. Methods To elucidate the glomerular cell–specific gene expression changes in
diabetic kidney disease, we performed scRNA-seq analysis of isolated glomerular cells from
streptozotocin-induced diabetic endothelial nitric oxide synthase (eNOS)–deficient …