Hemin-induced activation of the thioredoxin gene by Nrf2: a differential regulation of the antioxidant responsive element by a switch of its binding factors
YC Kim, H Masutani, Y Yamaguchi, K Itoh… - Journal of Biological …, 2001 - jbc.org
Thioredoxin plays an important role in various cellular processes through redox regulation.
Here, we have demonstrated that thioredoxin expression is transcriptionally induced in K562
cells by hemin (ferriprotoporphyrin IX) through activation of a regulatory region positioned
from− 452 to− 420 bp of the thioredoxin gene. Insertion of a mutation in the antioxidant
responsive element (ARE)/AP-1 consensus binding sequence in this region abolished the
response to hemin. With electrophoretic mobility shift and DNA affinity assays, we have …
Here, we have demonstrated that thioredoxin expression is transcriptionally induced in K562
cells by hemin (ferriprotoporphyrin IX) through activation of a regulatory region positioned
from− 452 to− 420 bp of the thioredoxin gene. Insertion of a mutation in the antioxidant
responsive element (ARE)/AP-1 consensus binding sequence in this region abolished the
response to hemin. With electrophoretic mobility shift and DNA affinity assays, we have …