Surgical injury to the mouse pancreas through ligation of the pancreatic duct as a model for endocrine and exocrine reprogramming and proliferation
S De Groef, G Leuckx, N Van Gassen, W Staels… - JoVE (Journal of …, 2015 - jove.com
Expansion of pancreatic beta cells in vivo or ex vivo, or generation of beta cells by
differentiation from an embryonic or adult stem cell, can provide new expandable sources of
beta cells to alleviate the donor scarcity in human islet transplantation as therapy for
diabetes. Although recent advances have been made towards this aim, mechanisms that
regulate beta cell expansion and differentiation from a stem/progenitor cell remain to be
characterized. Here, we describe a protocol for an injury model in the adult mouse pancreas …
differentiation from an embryonic or adult stem cell, can provide new expandable sources of
beta cells to alleviate the donor scarcity in human islet transplantation as therapy for
diabetes. Although recent advances have been made towards this aim, mechanisms that
regulate beta cell expansion and differentiation from a stem/progenitor cell remain to be
characterized. Here, we describe a protocol for an injury model in the adult mouse pancreas …