Safe harbours for the integration of new DNA in the human genome

M Sadelain, EP Papapetrou, FD Bushman - Nature Reviews Cancer, 2012 - nature.com
Nature Reviews Cancer, 2012nature.com
Interactions between newly integrate DNA and the host genome limit the reliability and
safety of transgene integration for therapeutic cell engineering and other applications.
Although targeted gene delivery has made considerable progress, the question of where to
insert foreign sequences in the human genome to maximize safety and efficacy has received
little attention. In this Opinion article, we discuss' genomic safe harbours'—chromosomal
locations where therapeutic transgenes can integrate and function in a predictable manner …
Abstract
Interactions between newly integrate DNA and the host genome limit the reliability and safety of transgene integration for therapeutic cell engineering and other applications. Although targeted gene delivery has made considerable progress, the question of where to insert foreign sequences in the human genome to maximize safety and efficacy has received little attention. In this Opinion article, we discuss 'genomic safe harbours' — chromosomal locations where therapeutic transgenes can integrate and function in a predictable manner without perturbing endogenous gene activity and promoting cancer.
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