Determining long-range chromatin interactions for selected genomic sites using 4C-seq technology: from fixation to computation

E Splinter, E de Wit, HJG van de Werken, P Klous… - Methods, 2012 - Elsevier
E Splinter, E de Wit, HJG van de Werken, P Klous, W de Laat
Methods, 2012Elsevier
Chromosome Conformation Capture (3C) and 3C-based technologies are constantly
evolving in order to probe nuclear organization with higher depth and resolution. One such
method is 4C-technology that allows the investigation of the nuclear environment of a locus
of choice. The use of Illumina next generation sequencing as a detection platform for the
analysis of 4C data has further improved the sensitivity and resolution of this method. Here
we provide a step-by-step protocol for 4C-seq, describing the procedure from the initial …
Chromosome Conformation Capture (3C) and 3C-based technologies are constantly evolving in order to probe nuclear organization with higher depth and resolution. One such method is 4C-technology that allows the investigation of the nuclear environment of a locus of choice. The use of Illumina next generation sequencing as a detection platform for the analysis of 4C data has further improved the sensitivity and resolution of this method. Here we provide a step-by-step protocol for 4C-seq, describing the procedure from the initial template preparation until the final data analysis, interchanged with background information and considerations.
Elsevier