Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells

PM Chaudhary, IB Roninson - Cell, 1991 - Elsevier
PM Chaudhary, IB Roninson
Cell, 1991Elsevier
Hem atopoietic stem cells show reduced staining with a mitochondrial fluorescent dye,
rhodamine 123 (Rh123), which was supposed to indicate decreased mitochondrial activity
in these cells. Rh123 and several other fluorescent dyes are substrates for transport
medlated by P-glycoprotein (P-gp), an efflux pump responsible for multidrug resistance in
tumor cells. We have found that staining of human bone marrow cells with fluorescent dyes
is potentiated by P-gp inhibitors and inversely correlated with P-gp expression. P-gp is …
Abstract
Hem atopoietic stem cells show reduced staining with a mitochondrial fluorescent dye, rhodamine 123 (Rh123), which was supposed to indicate decreased mitochondrial activity in these cells. Rh123 and several other fluorescent dyes are substrates for transport medlated by P-glycoprotein (P-gp), an efflux pump responsible for multidrug resistance in tumor cells. We have found that staining of human bone marrow cells with fluorescent dyes is potentiated by P-gp inhibitors and inversely correlated with P-gp expression. P-gp is expressed in practically all hematopoietic progenitor cells, including long-term culture-initiating cells. The highest levels of P-gp among the progenitors are associated with cells displaying characteristics of pluripotent stem cells. These results have implications for stem cell purification and bone marrow resistance to cancer chemotherapy.
Elsevier