Assaying double‐strand break repair pathway choice in mammalian cells using a targeted endonuclease or the RAG recombinase
DM Weinstock, K Nakanishi, HR Helgadottir… - Methods in …, 2006 - Elsevier
DNA damage repair is essential for the maintenance of genetic integrity in all organisms.
Unrepaired or imprecisely repaired DNA can lead to mutagenesis, cell death, or malignant
transformation. DNA damage in the form of double‐strand breaks (DSBs) can occur as a
result of both exogenous insults, such as ionizing radiation and drug therapies, and normal
metabolic processes including V (D) J recombination. Mammalian cells have multiple
pathways for repairing DSBs, including nonhomologous end‐joining (NHEJ), homologous …
Unrepaired or imprecisely repaired DNA can lead to mutagenesis, cell death, or malignant
transformation. DNA damage in the form of double‐strand breaks (DSBs) can occur as a
result of both exogenous insults, such as ionizing radiation and drug therapies, and normal
metabolic processes including V (D) J recombination. Mammalian cells have multiple
pathways for repairing DSBs, including nonhomologous end‐joining (NHEJ), homologous …