[HTML][HTML] Catalytic activities of mammalian epoxide hydrolases with cis and trans fatty acid epoxides relevant to skin barrier function

H Yamanashi, WE Boeglin, C Morisseau… - Journal of lipid …, 2018 - ASBMB
Lipoxygenase (LOX)-catalyzed oxidation of the essential fatty acid, linoleate, represents a
vital step in construction of the mammalian epidermal permeability barrier. Analysis of
epidermal lipids indicates that linoleate is converted to a trihydroxy derivative by hydrolysis
of an epoxy-hydroxy precursor. We evaluated different epoxide hydrolase (EH) enzymes in
the hydrolysis of skin-relevant fatty acid epoxides and compared the products to those of
acid-catalyzed hydrolysis. In the absence of enzyme, exposure to pH 5 or pH 6 at 37° C for …