Direct intrathecal drug delivery in mice for detecting in vivo effects of cGMP on pain processing
R Lu, A Schmidtko - Guanylate Cyclase and Cyclic GMP: Methods and …, 2013 - Springer
R Lu, A Schmidtko
Guanylate Cyclase and Cyclic GMP: Methods and Protocols, 2013•SpringerIntrathecal delivery of drugs is an important method in pain research in order to investigate
pain-relevant effects in the spinal cord in vivo. Here, we describe a method of intrathecal
drug delivery by direct lumbar puncture in mice. The procedure does not require surgery, is
rapidly performed, and does not produce neurological deficits. If cGMP analogs are injected,
a state of transient hindpaw hypersensitivity can be induced which is quantifiable by
measurement of hindpaw withdrawal latency in response to mechanical stimulation.
pain-relevant effects in the spinal cord in vivo. Here, we describe a method of intrathecal
drug delivery by direct lumbar puncture in mice. The procedure does not require surgery, is
rapidly performed, and does not produce neurological deficits. If cGMP analogs are injected,
a state of transient hindpaw hypersensitivity can be induced which is quantifiable by
measurement of hindpaw withdrawal latency in response to mechanical stimulation.
Abstract
Intrathecal delivery of drugs is an important method in pain research in order to investigate pain-relevant effects in the spinal cord in vivo. Here, we describe a method of intrathecal drug delivery by direct lumbar puncture in mice. The procedure does not require surgery, is rapidly performed, and does not produce neurological deficits. If cGMP analogs are injected, a state of transient hindpaw hypersensitivity can be induced which is quantifiable by measurement of hindpaw withdrawal latency in response to mechanical stimulation.
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