In vivo, the activity of antibodies relies critically on properties of both the variable domain,
responsible for antigen recognition, and the constant domain, responsible for innate immune
recognition. Here, we describe a flexible, microsphere-based array format for capturing
information about both functional ends of disease-specific antibodies from complex,
polyclonal clinical serum samples. Using minimal serum, we demonstrate IgG subclass
profiling of multiple antibody specificities. We further capture and determine the subclass of …