A diverse lipid antigen–specific TCR repertoire is clonally expanded during active tuberculosis

WS DeWitt, KKQ Yu, DB Wilburn… - The Journal of …, 2018 - journals.aai.org
WS DeWitt, KKQ Yu, DB Wilburn, A Sherwood, M Vignali, CL Day, TJ Scriba, HS Robins…
The Journal of Immunology, 2018journals.aai.org
Human T cells that recognize lipid Ags presented by highly conserved CD1 proteins often
express semi-invariant TCRs, but the true diversity of lipid Ag–specific TCRs remains
unknown. We use CD1b tetramers and high-throughput immunosequencing to analyze
thousands of TCRs from ex vivo–sorted or in vitro–expanded T cells specific for the
mycobacterial lipid Ag, glucose monomycolate. Our results reveal a surprisingly diverse
repertoire resulting from editing of germline-encoded gene rearrangements analogous to …
Abstract
Human T cells that recognize lipid Ags presented by highly conserved CD1 proteins often express semi-invariant TCRs, but the true diversity of lipid Ag–specific TCRs remains unknown. We use CD1b tetramers and high-throughput immunosequencing to analyze thousands of TCRs from ex vivo–sorted or in vitro–expanded T cells specific for the mycobacterial lipid Ag, glucose monomycolate. Our results reveal a surprisingly diverse repertoire resulting from editing of germline-encoded gene rearrangements analogous to MHC-restricted TCRs. We used a distance-based metric (TCRDist) to show how this diverse TCR repertoire builds upon previously reported conserved motifs by including subject-specific TCRs. In a South African cohort, we show that TCRDist can identify clonal expansion of diverse glucose monomycolate–specific TCRs and accurately distinguish patients with active tuberculosis from control subjects. These data suggest that similar mechanisms govern the selection and expansion of peptide and lipid Ag–specific T cells despite the nonpolymorphic nature of CD1.
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