Embryonic mouse spinal cord motor neuron hybrid cells
EF Salazar-Grueso, S Kim, H Kim - Neuroreport, 1991 - journals.lww.com
STUDIES of motor neurons are difficult because of limitations in their isolation and culture.
One solution is to produce clonal neural hybrid cells that can express motor neuron
characteristics; we fused an aminopterin-sensitive and neomycin-resistant mouse
neuroblastoma cell line to isolated embryonic mouse spinal cord motor neurons. Several
hybrid neuron cell lines expressing high levels of choline acetyltransferase (CHAT) enzyme
activity were found. These were cloned and clones with high CHAT activity isolated. The …
One solution is to produce clonal neural hybrid cells that can express motor neuron
characteristics; we fused an aminopterin-sensitive and neomycin-resistant mouse
neuroblastoma cell line to isolated embryonic mouse spinal cord motor neurons. Several
hybrid neuron cell lines expressing high levels of choline acetyltransferase (CHAT) enzyme
activity were found. These were cloned and clones with high CHAT activity isolated. The …