Detection of T-cell degranulation: CD107a and b
MR Betts, RA Koup - Methods in cell biology, 2004 - Elsevier
Publisher Summary This chapter describes an assay that helps bridge the gap between
measurement of CD8+ T-cell cytolytic activity and the identity of the effector CD8+ T cells.
Cytotoxic CD8+ T lymphocytes mediate the killing of target cells via two major pathways:(1)
perforin-granzyme-mediated activation of apoptosis and (2) fas–fas ligand–mediated
induction of apoptosis. Induction of these pathways depends on the release of cytolytic
granules from the responding CD8+ T cells. Degranulation is a prerequisite to perforin …
measurement of CD8+ T-cell cytolytic activity and the identity of the effector CD8+ T cells.
Cytotoxic CD8+ T lymphocytes mediate the killing of target cells via two major pathways:(1)
perforin-granzyme-mediated activation of apoptosis and (2) fas–fas ligand–mediated
induction of apoptosis. Induction of these pathways depends on the release of cytolytic
granules from the responding CD8+ T cells. Degranulation is a prerequisite to perforin …