Introduction of genes into primary murine splenic B cells using retrovirus vectors
KI Lin, K Calame - B Cell Protocols, 2004 - Springer
KI Lin, K Calame
B Cell Protocols, 2004•SpringerPrimary murine splenic B cells can be cultured ex vivo and, following treatment with LPS,
cytokines and/or CD40L proliferate, and undergo class switch recombination and terminal
differentiation to become immunoglobulin-secreting plasmacytic cells. Methods are
described here for introducing genes, encoding either normal or blocking forms of
experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it
possible to study the effects of these proteins on late stages of B-cell development, including …
cytokines and/or CD40L proliferate, and undergo class switch recombination and terminal
differentiation to become immunoglobulin-secreting plasmacytic cells. Methods are
described here for introducing genes, encoding either normal or blocking forms of
experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it
possible to study the effects of these proteins on late stages of B-cell development, including …
Abstract
Primary murine splenic B cells can be cultured ex vivo and, following treatment with LPS, cytokines and/or CD40L proliferate, and undergo class switch recombination and terminal differentiation to become immunoglobulin-secreting plasmacytic cells. Methods are described here for introducing genes, encoding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study the effects of these proteins on late stages of B-cell development, including proliferation, class switch recombination, and plasmacytic differentiation.
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