[HTML][HTML] A novel approach to recovery of function of mutant proteins by slowing down translation
AB Meriin, M Mense, JD Colbert, F Liang… - Journal of Biological …, 2012 - ASBMB
Protein homeostasis depends on a balance of translation, folding, and degradation. Here,
we demonstrate that mild inhibition of translation results in a dramatic and disproportional
reduction in production of misfolded polypeptides in mammalian cells, suggesting an
improved folding of newly synthesized proteins. Indeed, inhibition of translation elongation,
which slightly attenuated levels of a copepod GFP mutant protein, significantly enhanced its
function. In contrast, inhibition of translation initiation had minimal effects on copepod GFP …
we demonstrate that mild inhibition of translation results in a dramatic and disproportional
reduction in production of misfolded polypeptides in mammalian cells, suggesting an
improved folding of newly synthesized proteins. Indeed, inhibition of translation elongation,
which slightly attenuated levels of a copepod GFP mutant protein, significantly enhanced its
function. In contrast, inhibition of translation initiation had minimal effects on copepod GFP …