Messenger RNA interactions in the decoding center control the rate of translocation
PK Khade, S Joseph - Nature structural & molecular biology, 2011 - nature.com
PK Khade, S Joseph
Nature structural & molecular biology, 2011•nature.comDuring protein synthesis, mRNA and tRNAs are iteratively translocated by the ribosome.
Precisely what molecular event is rate limiting for translocation is not known. Here we show
that disruption of the interactions between the A-site codon and the ribosome accelerates
translocation, suggesting that the release of the mRNA from the decoding center of the
ribosome is the rate-limiting step of translocation. These results provide insight into the
molecular mechanism of translocation.
Precisely what molecular event is rate limiting for translocation is not known. Here we show
that disruption of the interactions between the A-site codon and the ribosome accelerates
translocation, suggesting that the release of the mRNA from the decoding center of the
ribosome is the rate-limiting step of translocation. These results provide insight into the
molecular mechanism of translocation.
Abstract
During protein synthesis, mRNA and tRNAs are iteratively translocated by the ribosome. Precisely what molecular event is rate limiting for translocation is not known. Here we show that disruption of the interactions between the A-site codon and the ribosome accelerates translocation, suggesting that the release of the mRNA from the decoding center of the ribosome is the rate-limiting step of translocation. These results provide insight into the molecular mechanism of translocation.
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