Purification and comparative assay of human mitochondrial single-stranded DNA-binding protein
GL Ciesielski, FA Rosado-Ruiz, LS Kaguni - Mitochondrial DNA: Methods …, 2016 - Springer
GL Ciesielski, FA Rosado-Ruiz, LS Kaguni
Mitochondrial DNA: Methods and Protocols, 2016•SpringerThe mitochondrial single-stranded DNA-binding protein (mtSSB) coordinates the function of
replisome components at the mitochondrial replication fork. In recent years, it has been
demonstrated that mtSSB stimulates the activities of DNA polymerase γ (Pol γ) and
mitochondrial DNA (mtDNA) helicase in a concentration-dependent manner. Here we
present a new approach to purify the human mtSSB and our standard assays to evaluate its
biochemical properties, including a Gel Mobility Shift Assay (GMSA) to assess single …
replisome components at the mitochondrial replication fork. In recent years, it has been
demonstrated that mtSSB stimulates the activities of DNA polymerase γ (Pol γ) and
mitochondrial DNA (mtDNA) helicase in a concentration-dependent manner. Here we
present a new approach to purify the human mtSSB and our standard assays to evaluate its
biochemical properties, including a Gel Mobility Shift Assay (GMSA) to assess single …
Abstract
The mitochondrial single-stranded DNA-binding protein (mtSSB) coordinates the function of replisome components at the mitochondrial replication fork. In recent years, it has been demonstrated that mtSSB stimulates the activities of DNA polymerase γ (Pol γ) and mitochondrial DNA (mtDNA) helicase in a concentration-dependent manner. Here we present a new approach to purify the human mtSSB and our standard assays to evaluate its biochemical properties, including a Gel Mobility Shift Assay (GMSA) to assess single-stranded DNA (ssDNA) binding activity, and an assay to assess SSB stimulation of Pol γ activity.
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