The kidney of the zebrafish shares many features with other vertebrate kidneys including the
human kidney. Similar cell types and shared developmental and patterning mechanisms
make the zebrafish pronephros a valuable model for kidney organogenesis. Here we review
recent advances in studies of zebrafish pronephric development and provide experimental
protocols to analyze kidney cell types and structures, measure nephron function, live image
kidney cells in vivo, and probe mechanisms of kidney regeneration after injury.