Mispairing C57BL/6 substrains of genetically engineered mice and wild-type controls can lead to confounding results as it did in studies of JNK2 in acetaminophen …

M Bourdi, JS Davies, LR Pohl - Chemical research in toxicology, 2011 - ACS Publications
M Bourdi, JS Davies, LR Pohl
Chemical research in toxicology, 2011ACS Publications
C57BL/6 mice are widely used in biomedical research for the background of genetically
engineered mice (GEM) and wild-type controls with the belief that the genetic background of
GEM and control mice differ significantly by only one or more altered genes. This principle,
however, does have limitations due in part to the existence of multiple substrains of C57BL/6
mice that should not be used interchangeably as they can differ both genetically and
phenotypically. We show here that these mispairings do occur frequently and can lead to …
C57BL/6 mice are widely used in biomedical research for the background of genetically engineered mice (GEM) and wild-type controls with the belief that the genetic background of GEM and control mice differ significantly by only one or more altered genes. This principle, however, does have limitations due in part to the existence of multiple substrains of C57BL/6 mice that should not be used interchangeably as they can differ both genetically and phenotypically. We show here that these mispairings do occur frequently and can lead to inaccurate and conflicting findings.
ACS Publications