Comparison of different standards for real-time PCR-based absolute quantification

S Dhanasekaran, TM Doherty, J Kenneth… - Journal of …, 2010 - Elsevier
Quantitative real-time PCR (qPCR) is a powerful tool used for both research and diagnostic,
which has the advantage, compared to relative quantification, of providing an absolute copy
number for a particular target. However, reliable standards are essential for qPCR. In this
study, we have compared four types of commonly-used standards—PCR products (with and
without purification) and cloned target sequences (circular and linear plasmid) for their
stability during storage (using percentage of variance in copy numbers, PCR efficiency and …