[HTML][HTML] The degradation of apolipoprotein B100 is mediated by the ubiquitin-proteasome pathway and involves heat shock protein 70
EA Fisher, M Zhou, DM Mitchell, X Wu, S Omura… - Journal of Biological …, 1997 - ASBMB
Apolipoprotein B (apoB) is the major protein component of atherogenic lipoproteins of
hepatic origin. In HepG2 cells, the standard cell culture model of human hepatic lipoprotein
metabolism, there is a limited availability of core lipids in the endoplasmic reticulum for
association with nascent apoB. Under these conditions, apoB is partially translocated,
interacts with cytosolic Hsp70, and undergoes rapid degradation. We show that increasing
the expression of Hsp70 in HepG2 cells promotes apoB degradation. In addition, apoB is …
hepatic origin. In HepG2 cells, the standard cell culture model of human hepatic lipoprotein
metabolism, there is a limited availability of core lipids in the endoplasmic reticulum for
association with nascent apoB. Under these conditions, apoB is partially translocated,
interacts with cytosolic Hsp70, and undergoes rapid degradation. We show that increasing
the expression of Hsp70 in HepG2 cells promotes apoB degradation. In addition, apoB is …