[HTML][HTML] A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A
E Ogris, X Du, KC Nelson, EK Mak, XX Yu… - Journal of Biological …, 1999 - ASBMB
Carboxymethylation of proteins is a highly conserved means of regulation in eukaryotic
cells. The protein phosphatase 2A (PP2A) catalytic (C) subunit is reversibly methylated at its
carboxyl terminus by specific methyltransferase and methylesterase enzymes which have
been purified, but not cloned. Carboxymethylation affects PP2A activity and varies during the
cell cycle. Here, we report that substitution of glutamine for either of two putative active site
histidines in the PP2A C subunit results in inactivation of PP2A and formation of stable …
cells. The protein phosphatase 2A (PP2A) catalytic (C) subunit is reversibly methylated at its
carboxyl terminus by specific methyltransferase and methylesterase enzymes which have
been purified, but not cloned. Carboxymethylation affects PP2A activity and varies during the
cell cycle. Here, we report that substitution of glutamine for either of two putative active site
histidines in the PP2A C subunit results in inactivation of PP2A and formation of stable …