[PDF][PDF] Expression of inflammatory genes in the colon of ulcerative colitis patients varies with activity both at the mRNA and protein level

R Verma, N Verma, J Paul - Eur Cytokine Netw, 2013 - researchgate.net
Eur Cytokine Netw, 2013researchgate.net
Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the gastrointestinal
tract involving aberrant activation of innate and adaptive immune responses. We aimed to
study the expression profiles of the susceptibility gene Nod1 and selected pro-and anti-
inflammatory cytokines during different stages of UC. Methods: 65 patients with mild, severe
or remission stage UC, and 22 normal colon mucosal biopsies from control individuals were
included in the study for measuring the expression of nucleotide binding oligomerization 1 …
Abstract
Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the gastrointestinal tract involving aberrant activation of innate and adaptive immune responses. We aimed to study the expression profiles of the susceptibility gene Nod1 and selected pro-and anti-inflammatory cytokines during different stages of UC.
Methods: 65 patients with mild, severe or remission stage UC, and 22 normal colon mucosal biopsies from control individuals were included in the study for measuring the expression of nucleotide binding oligomerization 1 (Nod1) and related pro-and anti-inflammatory cytokines using quantitative reverse transcription-PCR (qRT-PCR). mRNA expression levels were then correlated with severity of disease. In order to check their expression at the protein level, immunohistochemistry (IHC) was performed using Nod1, TNF-, IFN-, IL-17, IL-23 and IL-13 antibodies.
Results: Significant increases in Nod1 expression with simultaneous increases in pro-inflammatory cytokines TNF-,
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