[HTML][HTML] Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV

EP Brown, JA Weiner, S Lin, H Natarajan… - Journal of …, 2018 - Elsevier
Abstract The Fc Array is a multiplexed assay that assesses the Fc domain characteristics of
antigen-specific antibodies with the potential to evaluate up to 500 antigen specificities
simultaneously. Antigen-specific antibodies are captured on antigen-conjugated beads and
their functional capacity is probed via an array of Fc-binding proteins including antibody
subclassing reagents, Fcγ receptors, complement proteins, and lectins. Here we present the
results of the optimization and formal qualification of the Fc Array, performed in compliance …