[PDF][PDF] In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria
Croatian medical journal, 2015•hrcak.srce.hr
Aim To investigate whether the sex-dependent expression of hepatic and renal oxalate
transporter sat-1 (Slc26a1) changes in a rat model of ethylene glycol (EG)-induced
hyperoxaluria. Methods Rats were given tap water (12 males and 12 females; controls) or
EG (12 males and 12 females; 0.75% v/v in tap water) for one month. Oxaluric state was
confirmed by biochemical parameters in blood plasma, urine, and tissues. Expression of sat-
1 and rate-limiting enzymes of oxalate synthesis, alcohol dehydrogenase 1 (Adh1) and …
transporter sat-1 (Slc26a1) changes in a rat model of ethylene glycol (EG)-induced
hyperoxaluria. Methods Rats were given tap water (12 males and 12 females; controls) or
EG (12 males and 12 females; 0.75% v/v in tap water) for one month. Oxaluric state was
confirmed by biochemical parameters in blood plasma, urine, and tissues. Expression of sat-
1 and rate-limiting enzymes of oxalate synthesis, alcohol dehydrogenase 1 (Adh1) and …
Aim To investigate whether the sex-dependent expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) changes in a rat model of ethylene glycol (EG)-induced hyperoxaluria.
Methods Rats were given tap water (12 males and 12 females; controls) or EG (12 males and 12 females; 0.75% v/v in tap water) for one month. Oxaluric state was confirmed by biochemical parameters in blood plasma, urine, and tissues. Expression of sat-1 and rate-limiting enzymes of oxalate synthesis, alcohol dehydrogenase 1 (Adh1) and hydroxy-acid oxidase 1 (Hao1), was determined by immunocytochemistry (protein) and/or real time reverse transcription polymerase chain reaction (mRNA).
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