Impaired mucus detachment disrupts mucociliary transport in a piglet model of cystic fibrosis

MJ Hoegger, AJ Fischer, JD McMenimen… - Science, 2014 - science.org
MJ Hoegger, AJ Fischer, JD McMenimen, LS Ostedgaard, AJ Tucker, MA Awadalla…
Science, 2014science.org
Lung disease in people with cystic fibrosis (CF) is initiated by defective host defense that
predisposes airways to bacterial infection. Advanced CF is characterized by a deficit in
mucociliary transport (MCT), a process that traps and propels bacteria out of the lungs, but
whether this deficit occurs first or is secondary to airway remodeling has been unclear. To
assess MCT, we tracked movement of radiodense microdisks in airways of newborn piglets
with CF. Cholinergic stimulation, which elicits mucus secretion, substantially reduced …
Lung disease in people with cystic fibrosis (CF) is initiated by defective host defense that predisposes airways to bacterial infection. Advanced CF is characterized by a deficit in mucociliary transport (MCT), a process that traps and propels bacteria out of the lungs, but whether this deficit occurs first or is secondary to airway remodeling has been unclear. To assess MCT, we tracked movement of radiodense microdisks in airways of newborn piglets with CF. Cholinergic stimulation, which elicits mucus secretion, substantially reduced microdisk movement. Impaired MCT was not due to periciliary liquid depletion; rather, CF submucosal glands secreted mucus strands that remained tethered to gland ducts. Inhibiting anion secretion in non-CF airways replicated CF abnormalities. Thus, impaired MCT is a primary defect in CF, suggesting that submucosal glands and tethered mucus may be targets for early CF treatment.
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