Multidrug resistance mediated by overexpression of P-glycoprotein in human osteosarcoma in vivo.

R Suto, Y Abe, M Nakamura… - International …, 1998 - spandidos-publications.com
R Suto, Y Abe, M Nakamura, Y Ohnishi, M Yoshimura, YH Lee, T Imanishi, H Yamazaki…
International journal of oncology, 1998spandidos-publications.com
We examined the expression levels of P-glycoprotein (P-Gp)/the human multidrug
resistance gene (MDR1) and in vivo chemosensitivity in the 7 osteosarcoma xenografts.
Three of seven (43%) osteosarcoma xenografts expressed MDR1 by reverse transcriptase-
polymerase chain reaction (RT-PCR). The OSS-516R xenograft selected with vincristine
(VCR) from the MDR1-negative xenograft OSS-516, which was sensitive to VCR and
doxorubicin (DOX), acquired cross-resistance to DOX. In the OSS-516R, RT-PCR assay …
Abstract
We examined the expression levels of P-glycoprotein (P-Gp)/the human multidrug resistance gene (MDR1) and in vivo chemosensitivity in the 7 osteosarcoma xenografts. Three of seven (43%) osteosarcoma xenografts expressed MDR1 by reverse transcriptase-polymerase chain reaction (RT-PCR). The OSS-516R xenograft selected with vincristine (VCR) from the MDR1-negative xenograft OSS-516, which was sensitive to VCR and doxorubicin (DOX), acquired cross-resistance to DOX. In the OSS-516R, RT-PCR assay showed definite MDR1 expression and immunohistochemical analysis demonstrated P-Gp-positive tumor cells. These results suggest that P-Gp/MDR1 overexpression is related to multidrug resistance in human osteosarcoma in vivo.
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