Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin
K Rader, RA Orlando, X Lou… - Journal of the American …, 2000 - journals.lww.com
Ankyrin-repeat family A protein (ANKRA) is a novel protein that interacts directly and
specifically with the cytoplasmic tail of megalin in the yeast two-hybrid system and
glutathione-S-transferase pull-down assays. ANKRA has three ankyrin repeats and shows
61% overall homology to regulatory factor X, ankyrin repeat-containing protein. Mapping
studies show that the three ankyrin repeats and C-terminus of ANKRA are required for
binding to a unique juxtamembrane, 19-amino acid sequence on the megalin tail. Point …
specifically with the cytoplasmic tail of megalin in the yeast two-hybrid system and
glutathione-S-transferase pull-down assays. ANKRA has three ankyrin repeats and shows
61% overall homology to regulatory factor X, ankyrin repeat-containing protein. Mapping
studies show that the three ankyrin repeats and C-terminus of ANKRA are required for
binding to a unique juxtamembrane, 19-amino acid sequence on the megalin tail. Point …