Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose
KC O'connor, H Song, N Rosenzweig, DA Jansen - Biotechnology letters, 2003 - Springer
KC O'connor, H Song, N Rosenzweig, DA Jansen
Biotechnology letters, 2003•SpringerThe stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on
different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to
45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-
vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture
plastic and collagen I; and 59%±7% on Matrigel. Lipid volume surpassed 4.5× 10 3 μm 3
cell− 1 for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident …
different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to
45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-
vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture
plastic and collagen I; and 59%±7% on Matrigel. Lipid volume surpassed 4.5× 10 3 μm 3
cell− 1 for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident …
Abstract
The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% ± 7% on Matrigel. Lipid volume surpassed 4.5×103 μm3 cell−1 for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3×103 μm2 cell−1. These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.
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