Agouti C57BL/6N embryonic stem cells for mouse genetic resources
SJ Pettitt, Q Liang, XY Rairdan, JL Moran, HM Prosser… - Nature …, 2009 - nature.com
Nature methods, 2009•nature.com
We report the characterization of a highly germline competent C57BL/6N mouse embryonic
stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was
restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells
provide a robust foundation for large-scale mouse knockout programs that aim to provide a
public resource of targeted mutations in the C57BL/6 genetic background.
stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was
restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells
provide a robust foundation for large-scale mouse knockout programs that aim to provide a
public resource of targeted mutations in the C57BL/6 genetic background.
Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
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