[HTML][HTML] Characterization of pancreatic transcription factor Pdx-1 binding sites using promoter microarray and serial analysis of chromatin occupancy
DM Keller, S McWeeney, A Arsenlis, J Drouin… - Journal of biological …, 2007 - ASBMB
The homeobox transcription factor Pdx-1 is necessary for pancreas organogenesis and beta
cell function, however, most Pdx-1-regulated genes are unknown. To further the
understanding of Pdx-1 in beta cell biology, we have characterized its genomic targets in
NIT-1 cells, a mouse insulinoma cell line. To identify novel targets, we developed a
microarray that includes traditional promoters as well as non-coding conserved elements,
micro-RNAs, and elements identified through an unbiased approach termed serial analysis …
cell function, however, most Pdx-1-regulated genes are unknown. To further the
understanding of Pdx-1 in beta cell biology, we have characterized its genomic targets in
NIT-1 cells, a mouse insulinoma cell line. To identify novel targets, we developed a
microarray that includes traditional promoters as well as non-coding conserved elements,
micro-RNAs, and elements identified through an unbiased approach termed serial analysis …