[HTML][HTML] Anti-inflammatory apoA-I-mimetic peptides bind oxidized lipids with much higher affinity than human apoA-I
BJ Van Lenten, AC Wagner, CL Jung, P Ruchala… - Journal of lipid …, 2008 - ASBMB
4F is an anti-inflammatory, apolipoprotein AI (apoA-I)-mimetic peptide that is active in vivo at
nanomolar concentrations in the presence of a large molar excess of apoA-I. Physiologic
concentrations (∼ 35 μM) of human apoA-I did not inhibit the production of LDL-induced
monocyte chemotactic activity by human aortic endothelial cell cultures, but adding
nanomolar concentrations of 4F in the presence of∼ 35 μM apoA-I significantly reduced this
inflammatory response. We analyzed lipid binding by surface plasmon resonance. The anti …
nanomolar concentrations in the presence of a large molar excess of apoA-I. Physiologic
concentrations (∼ 35 μM) of human apoA-I did not inhibit the production of LDL-induced
monocyte chemotactic activity by human aortic endothelial cell cultures, but adding
nanomolar concentrations of 4F in the presence of∼ 35 μM apoA-I significantly reduced this
inflammatory response. We analyzed lipid binding by surface plasmon resonance. The anti …