A myeloid-derived suppressor cell–mediated T-cell suppression assay for functional evaluation of immune cells in tumor-bearing mice
LJ Bayne, RH Vonderheide - Cold Spring Harbor Protocols, 2013 - cshprotocols.cshlp.org
LJ Bayne, RH Vonderheide
Cold Spring Harbor Protocols, 2013•cshprotocols.cshlp.orgThis protocol describes a procedure to evaluate the ability of Gr-1+ CD11b+ myeloid-derived
suppressor cells (MDSCs) isolated from the spleen or tumor of a tumor-bearing mouse to
suppress antigen-specific T-cell proliferation. MDSCs are cultured with responder
splenocytes from an OT-1 T-cell receptor transgenic mouse stimulated with cognate peptide
(OVA-257 peptide) or with an irrelevant, control peptide. This protocol spans a 4-d period.
The readout is T-cell proliferation by 3 [H]-thymidine incorporation during an 18-h pulse …
suppressor cells (MDSCs) isolated from the spleen or tumor of a tumor-bearing mouse to
suppress antigen-specific T-cell proliferation. MDSCs are cultured with responder
splenocytes from an OT-1 T-cell receptor transgenic mouse stimulated with cognate peptide
(OVA-257 peptide) or with an irrelevant, control peptide. This protocol spans a 4-d period.
The readout is T-cell proliferation by 3 [H]-thymidine incorporation during an 18-h pulse …
Abstract
This protocol describes a procedure to evaluate the ability of Gr-1+ CD11b+ myeloid-derived suppressor cells (MDSCs) isolated from the spleen or tumor of a tumor-bearing mouse to suppress antigen-specific T-cell proliferation. MDSCs are cultured with responder splenocytes from an OT-1 T-cell receptor transgenic mouse stimulated with cognate peptide (OVA-257 peptide) or with an irrelevant, control peptide. This protocol spans a 4-d period. The readout is T-cell proliferation by 3 [H]-thymidine incorporation during an 18-h pulse between Days 3 and 4.
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