[HTML][HTML] NF-κB/p65 antagonizes Nrf2-ARE pathway by depriving CBP from Nrf2 and facilitating recruitment of HDAC3 to MafK

GH Liu, J Qu, X Shen - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2008 - Elsevier
GH Liu, J Qu, X Shen
Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2008Elsevier
Constitutively activated NF-κB occurs in many inflammatory and tumor tissues. Does it
interfere with anti-inflammatory or anti-tumor signaling pathway? Here, we report that NF-κB
p65 subunit repressed the Nrf2-antioxidant response element (ARE) pathway at
transcriptional level. In the cells where NF-κB and Nrf2 were simultaneously activated, p65
unidirectionally antagonized the transcriptional activity of Nrf2. In the p65-overexpressing
cells, the ARE-dependent expression of heme oxygenase-1 was strongly suppressed …
Constitutively activated NF-κB occurs in many inflammatory and tumor tissues. Does it interfere with anti-inflammatory or anti-tumor signaling pathway? Here, we report that NF-κB p65 subunit repressed the Nrf2-antioxidant response element (ARE) pathway at transcriptional level. In the cells where NF-κB and Nrf2 were simultaneously activated, p65 unidirectionally antagonized the transcriptional activity of Nrf2. In the p65-overexpressing cells, the ARE-dependent expression of heme oxygenase-1 was strongly suppressed. However, p65 inhibited the ARE-driven gene transcription in a way that was independent of its own transcriptional activity. Two mechanisms were found to coordinate the p65-mediated repression of ARE: (1) p65 selectively deprives CREB binding protein (CBP) from Nrf2 by competitive interaction with the CH1-KIX domain of CBP, which results in inactivation of Nrf2. The inactivation depends on PKA catalytic subunit-mediated phosphorylation of p65 at S276. (2) p65 promotes recruitment of histone deacetylase 3 (HDAC3), the corepressor, to ARE by facilitating the interaction of HDAC3 with either CBP or MafK, leading to local histone hypoacetylation. This investigation revealed the participation of NF-κB p65 in the negative regulation of Nrf2-ARE signaling, and might provide a new insight into a possible role of NF-κB in suppressing the expression of anti-inflammatory or anti-tumor genes.
Elsevier