The transcription factor Gli3 regulates differentiation of fetal CD4–CD8– double-negative thymocytes
AL Hager-Theodorides, JT Dessens, SV Outram… - Blood, 2005 - ashpublications.org
AL Hager-Theodorides, JT Dessens, SV Outram, T Crompton
Blood, 2005•ashpublications.orgAbstract Glioblastoma 3 (Gli3) is a transcription factor involved in patterning and
oncogenesis. Here, we demonstrate a role for Gli3 in thymocyte development. Gli3 is
differentially expressed in fetal CD4–CD8–double-negative (DN) thymocytes and is most
highly expressed at the CD44+ CD25–DN (DN1) and CD44–CD25–(DN4) stages of
development but was not detected in adult thymocytes. Analysis of null mutants showed that
Gli3 is involved at the transitions from DN1 to CD44+ CD25+ DN (DN2) cell and from DN to …
oncogenesis. Here, we demonstrate a role for Gli3 in thymocyte development. Gli3 is
differentially expressed in fetal CD4–CD8–double-negative (DN) thymocytes and is most
highly expressed at the CD44+ CD25–DN (DN1) and CD44–CD25–(DN4) stages of
development but was not detected in adult thymocytes. Analysis of null mutants showed that
Gli3 is involved at the transitions from DN1 to CD44+ CD25+ DN (DN2) cell and from DN to …
Abstract
Glioblastoma 3 (Gli3) is a transcription factor involved in patterning and oncogenesis. Here, we demonstrate a role for Gli3 in thymocyte development. Gli3 is differentially expressed in fetal CD4–CD8– double-negative (DN) thymocytes and is most highly expressed at the CD44+ CD25– DN (DN1) and CD44–CD25– (DN4) stages of development but was not detected in adult thymocytes. Analysis of null mutants showed that Gli3 is involved at the transitions from DN1 to CD44+ CD25+ DN (DN2) cell and from DN to CD4+CD8+ double-positive (DP) cell. Gli3 is required for differentiation from DN to DP thymocyte, after pre–T-cell receptor (TCR) signaling but is not necessary for pre-TCR–induced proliferation or survival. The effect of Gli3 was dose dependent, suggesting its direct involvement in the transcriptional regulation of genes controlling T-cell differentiation during fetal development.
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